References on Ribosome Engineering, Translation, and Genetic Code Expansion
The following scientific references support research on bacterial ribosomes, mRNA translation, orthogonal translation systems, synthetic genetic code expansion, cell-free protein synthesis, ribosome assembly, and translation quality control.
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Noller, H. F. Evolution of protein synthesis from an RNA world. Cold Spring Harbor Perspect. Biol. 4, a003681 (2012).
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Wang, K., Neumann, H., Peak-Chew, S. Y. & Chin, J. W. Evolved orthogonal ribosomes enhance the efficiency of synthetic genetic code expansion. Nat. Biotechnol. 25, 770–777 (2007).
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Osterman, I. A., Evfratov, S. A., Sergiev, P. V. & Dontsova, O. A. Comparison of mRNA features affecting translation initiation and reinitiation. Nucleic Acids Res. 41, 474–486 (2013).
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Dorywalska, M. et al. Site-specific labeling of the ribosome for single-molecule spectroscopy. Nucleic Acids Res. 33, 182–189 (2005).
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Boyko, K. V., Bernstein, R. A., Kim, M. & Cate, J. H. D. Role of ribosomal protein bS1 in orthogonal mRNA start codon selection. Biochemistry 64, 710–718 (2025).
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Shimizu, Y. et al. Cell-free translation reconstituted with purified components. Nat. Biotechnol. 19, 751–755 (2001).
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Krupovic, M. & Koonin, E. V. The selfish ribosome. PLoS Biol. 24, e3003780 (2026).
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Huang, S. et al. Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly. Nat. Commun. 11, 2900 (2020).
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Fried, S. D., Schmied, W. H., Uttamapinant, C. & Chin, J. W. Ribosome subunit stapling for orthogonal translation in E. coli. Angew. Chem. Int. Edit. 54, 12791–12794 (2015).
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McGlincy, N. J. & Ingolia, N. T. Transcriptome-wide measurement of translation by ribosome profiling. Methods 126, 112–129 (2017).
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